TGFA Protein (AA 40-89) (hFc Tag)

Catalog No. ABIN7275716

Recombinant TGFA protein expressed in HEK-293 Cells.

Quick Overview for TGFA Protein (AA 40-89) (hFc Tag) (ABIN7275716)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 40-89

Purification tag / Conjugate: This TGFA protein is labelled with hFc Tag.

Purpose: Human TGF-alpha Protein

Sequence: Val40-Ala89

Characteristics: Recombinant Human TGF-alpha Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Val40-Ala89.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).,Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test).,Human TGF-alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 μM as determined in BLI assay (Gator® Prime).,The purity of Human TGF-alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris,100 mM NaCl ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-alpha

Background: Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice.

Molecular Weight: 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway