TGFA Protein (AA 39-88) (hFc Tag)

Catalog No. ABIN7275722

Recombinant TGFA protein expressed in HEK-293 Cells.

Quick Overview for TGFA Protein (AA 39-88) (hFc Tag) (ABIN7275722)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 39-88

Purification tag / Conjugate: This TGFA protein is labelled with hFc Tag.

Purpose: Mouse TGF-alpha Protein

Sequence: Val39-Ala88

Characteristics: Recombinant Mouse TGF-alpha Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Val39-Ala88.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse TGF-alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse TGF-alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-alpha

Background: Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice.

Molecular Weight: 32.9 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.

UniProt: P48030

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway