TLR3 Protein (AA 26-705) (His tag)

Catalog No. ABIN7275748

This Recombinant TLR3 protein is expressed in HEK-293 Cells.

Quick Overview for TLR3 Protein (AA 26-705) (His tag) (ABIN7275748)

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-705

Purification tag / Conjugate: This TLR3 protein is labelled with His tag.

Purpose: Mouse TLR3 Protein

Sequence: Thr26-Leu705

Characteristics: Recombinant Mouse TLR3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr26-Leu705.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse TLR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse TLR3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Alternative Name: TLR3

Background: TLR3 is expressed in the central nervous system (CNS), where it is required to control HSV-1, which spreads from the epithelium to the CNS via cranial nerves. TLR3 is also expressed in epithelial and dendritic cells, which apparently use TLR3-independent pathways to prevent further dissemination of HSV-1 and to provide resistance to other pathogens in TLR3-deficient patients. Human TLR3 appears to be redundant in host defense to most microbes but is vital for natural immunity to HSV-1 in the CNS, which suggests that neurotropic viruses have contributed to the evolutionary maintenance of TLR3.

Molecular Weight: 78.6 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Bis-Tris PAGE result.

UniProt: Q99MB1

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades