TNFRSF1A Protein (AA 30-211) (His tag)

Catalog No. ABIN7275755

Recombinant TNFRSF1A protein expressed in HEK-293 Cells.

Quick Overview for TNFRSF1A Protein (AA 30-211) (His tag) (ABIN7275755)

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 30-211

Purification tag / Conjugate: This TNFRSF1A protein is labelled with His tag.

Purpose: Cynomolgus TNFR1/CD120a/TNFRSF1A Protein

Sequence: Leu30-Thr211

Characteristics: Recombinant Cynomolgus TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Thr211.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus TNFR1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test).,Cynomolgus TNFR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus TNFR1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Alternative Name: TNFR1

Background: Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.

Molecular Weight: 21.62 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Bis-Tris PAGE result.

UniProt: A0A7N9CVF2

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway