TNFRSF1A Protein (His tag)

Catalog No. ABIN7198475

Product Details

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF1A protein is labelled with His tag.

Purpose: Recombinant Rat TNFR1/TNFRSF1A Protein (His Tag)(Active)

Sequence: Met1-Ala211

Characteristics: A DNA sequence encoding the rat TNFRSF1A (Met1-Ala211) was expressed with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.4-2 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Alternative Name: TNFR1/TNFRSF1A (TNFRSF1A Products)

Synonyms: CD120a Protein, FPF Protein, MS5 Protein, TBP1 Protein, TNF-R Protein, TNF-R-I Protein, TNF-R55 Protein, TNFAR Protein, TNFR1 Protein, TNFR1-d2 Protein, TNFR55 Protein, TNFR60 Protein, p55 Protein, p55-R Protein, p60 Protein, TNF-R1 Protein, TNF-alphaR1 Protein, TNFRI Protein, TNFRp55 Protein, TNFalpha-R1 Protein, Tnfr-2 Protein, Tnfr1 Protein, fpf Protein, tbp1 Protein, p55-r Protein, tnf-r Protein, tnfar Protein, tnfr1 Protein, cd120a Protein, tnfr55 Protein, tnfr60 Protein, xtnfr1 Protein, wu:fj65b10 Protein, zgc:85914 Protein, TNFR I Protein, TNFRSF1A Protein, TNFA Protein, cTNF Protein, TNF receptor superfamily member 1A Protein, tumor necrosis factor receptor superfamily, member 1a Protein, TNF receptor superfamily member 1A S homeolog Protein, tumor necrosis factor Protein, TNFRSF1A Protein, Tnfrsf1a Protein, tnfrsf1a.S Protein, tnfrsf1a Protein, TNF Protein

Background:

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

Synonym: TNFRSF1A;Tnfr-1;Tnfr1

Molecular Weight: 22.3 kDa

NCBI Accession: NP_037223

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway