DcR1 Protein (AA 24-236) (His tag)

Catalog No. ABIN7275773

Recombinant DcR1 protein expressed in HEK-293 Cells.

Quick Overview for DcR1 Protein (AA 24-236) (His tag) (ABIN7275773)

Target: DcR1 (TNFRSF10C) (Tumor Necrosis Factor Receptor Superfamily, Member 10c (TNFRSF10C))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-236

Purification tag / Conjugate: This DcR1 protein is labelled with His tag.

Purpose: Human TRAIL R3/DcR1 Protein

Sequence: Tyr24-Ala236

Characteristics: Recombinant Human TRAIL R3/DcR1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr24-Ala236.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human TRAIL R3, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).,Human TRAIL R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human TRAIL R3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DcR1 (TNFRSF10C) (Tumor Necrosis Factor Receptor Superfamily, Member 10c (TNFRSF10C))

Alternative Name: DcR1

Background: The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family, which mediates apoptosis by the extrinsic pathway. Up-regulation of decoy receptors, DcR1 and DcR2, may result in diminished binding of TRAIL to their functional receptors. DcR1 expression occurs in a subset of EC and may contribute to resistance to TRAIL-induced apoptosis.

Molecular Weight: 23.5 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.

UniProt: Q05D71

Pathways: Apoptosis