TNFRSF10B Protein (AA 56-182) (His-Avi Tag)

Catalog No. ABIN7275779

Recombinant TNFRSF10B protein expressed in HEK-293 Cells.

Quick Overview for TNFRSF10B Protein (AA 56-182) (His-Avi Tag) (ABIN7275779)

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 56-182

Purification tag / Conjugate: This TNFRSF10B protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human TRAIL R2/DR5/TNFRSF10B Protein

Sequence: Ile56-Glu182

Characteristics: Recombinant Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile56-Glu182.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TRAIL R2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TRAIL R2 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test).,Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, His Tag with the EC50 of 25.0ng/ml determined by ELISA.,Human TRAIL R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human TRAIL R2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TRAIL R2

Background: DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.

Molecular Weight: 17.2 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.

UniProt: O14763

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity