TNFRSF10B Protein (AA 53-180) (His tag)

Catalog No. ABIN7275784

This Recombinant TNFRSF10B protein is expressed in HEK-293 Cells.

Quick Overview for TNFRSF10B Protein (AA 53-180) (His tag) (ABIN7275784)

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 53-180

Purification tag / Conjugate: This TNFRSF10B protein is labelled with His tag.

Purpose: Mouse TRAIL R2/DR5/TNFRSF10B Protein

Sequence: Asn53-Lys180

Characteristics: Recombinant Mouse TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn53-Lys180.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse TRAIL R2, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.68 nM as determined in SPR assay (Biacore T200).,Mouse TRAIL R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse TRAIL R2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TRAIL R2

Background: DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.

Molecular Weight: 15.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

UniProt: Q9QZM4

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity