CRLF2 Protein (AA 23-231) (His tag)
Quick Overview for CRLF2 Protein (AA 23-231) (His tag) (ABIN7275801)
Target
See all CRLF2 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 23-231
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Purification tag / Conjugate
- This CRLF2 protein is labelled with His tag.
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Purpose
- Cynomolgus TSLPR Protein
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Sequence
- Gln23-Lys231
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Characteristics
- Recombinant Cynomolgus TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Lys231.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Cynomolgus TSLPR, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. See testing image for detail.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))
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Alternative Name
- TSLPR
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Background
- Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).
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Molecular Weight
- 25.42 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.
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NCBI Accession
- XP_045239630
Target
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