CRLF2 Protein (AA 25-231) (hFc Tag)

Catalog No. ABIN7275803

Recombinant CRLF2 protein expressed in HEK-293 Cells.

Quick Overview for CRLF2 Protein (AA 25-231) (hFc Tag) (ABIN7275803)

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-231

Purification tag / Conjugate: This CRLF2 protein is labelled with hFc Tag.

Purpose: Human TSLPR Protein

Sequence: Gly25-Lys231

Characteristics: Recombinant Human TSLPR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly25-Lys231.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human TSLP, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human TSLPR, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).,Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).,Immobilized Human TSLPR, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSLPR Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA.,Human TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP, His Tag with an affinity constant of 31.30 nM as determined in BLI assay (Gator® Prime).,The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Alternative Name: TSLPR

Background: Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).

Molecular Weight: 50.8 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.

UniProt: Q9HC73