CRLF2 Protein (AA 23-231) (His tag)

Catalog No. ABIN7275801

This Recombinant CRLF2 protein is produced in HEK-293 Cells.

Quick Overview for CRLF2 Protein (AA 23-231) (His tag) (ABIN7275801)

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-231

Purification tag / Conjugate: This CRLF2 protein is labelled with His tag.

Purpose: Cynomolgus TSLPR Protein

Sequence: Gln23-Lys231

Characteristics: Recombinant Cynomolgus TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Lys231.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).,Immobilized Cynomolgus TSLPR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA.,Cynomolgus TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus TSLPR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Alternative Name: TSLPR

Background: Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).

Molecular Weight: 25.42 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_045239630