CRLF2 Protein (AA 23-231) (His tag)

Catalog No. ABIN7275801

Product Details

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Protein Type: Recombinant

Protein Characteristics: AA 23-231

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This CRLF2 protein is labelled with His tag.

Purpose: Cynomolgus TSLPR Protein

Sequence: Gln23-Lys231

Characteristics: Recombinant Cynomolgus TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Lys231.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus TSLPR, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Alternative Name: TSLPR (CRLF2 Products)

Synonyms: CRLF2 Protein, CRL2 Protein, CRLF2Y Protein, TSLPR Protein, CRLM2 Protein, Ly114 Protein, Tpte2 Protein, Tslpr Protein, Crl2 Protein, cytokine receptor like factor 2 Protein, cytokine receptor-like factor 2 Protein, CRLF2 Protein, Crlf2 Protein

Background: Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).

Molecular Weight: 25.42 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_045239630