ULBP2 Protein (AA 26-217) (Biotin,His-Avi Tag)

Catalog No. ABIN7275816

This Recombinant ULBP2 protein is produced in HEK-293 Cells.

Quick Overview for ULBP2 Protein (AA 26-217) (Biotin,His-Avi Tag) (ABIN7275816)

Target: ULBP2 (UL16 Binding Protein 2 (ULBP2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-217

Purification tag / Conjugate: This ULBP2 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human ULBP-2 Protein

Sequence: Gly26-Ser217

Characteristics: Recombinant Biotinylated Human ULBP-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Ser217.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human ULBP-2, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test).,Biotinylated Human ULBP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.,The purity of Biotinylated Human ULBP-2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ULBP2 (UL16 Binding Protein 2 (ULBP2))

Alternative Name: ULBP-2

Background: ULBP2 expression was however linked to expression of mature CD57() NK cells. In particular, expression of ULBP2 was strongest on those NK cells that had evidence of recent activation and proliferation. We suggest that ULBP2 could be used to identify recently activated "mature" NK cells.

Molecular Weight: 24.6 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

UniProt: Q9BZM5