ULBP1 Protein (AA 26-215) (Biotin,His-Avi Tag)

Catalog No. ABIN7275818

Recombinant ULBP1 protein expressed in HEK-293 Cells.

Quick Overview for ULBP1 Protein (AA 26-215) (Biotin,His-Avi Tag) (ABIN7275818)

Target: ULBP1 (UL16 Binding Protein 1 (ULBP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-215

Purification tag / Conjugate: This ULBP1 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human ULBP-1 Protein

Sequence: Gly26-Pro215

Characteristics: Recombinant Biotinylated Human ULBP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Pro215.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA.,Biotinylated Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 20 mM PB, 0.5M NaCl, 0.1M L-arginine ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ULBP1 (UL16 Binding Protein 1 (ULBP1))

Alternative Name: ULBP-1

Background: Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.

Molecular Weight: 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.

UniProt: Q9BZM6

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process