ULBP1 Protein (His tag)

Catalog No. ABIN7317805

Product Details

Target: ULBP1 (UL16 Binding Protein 1 (ULBP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ULBP1 protein is labelled with His tag.

Purpose: Recombinant Human ULBP1/N2DL1 Protein (His Tag)(Active)

Sequence: Met 1-Gly 216

Characteristics: A DNA sequence encoding the mature form of human ULBP1 (NP_079494.1) (Met 1-Gly 216) was expressed, with a polyhistidine tag at the C-terminus.

Purity: > 98 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized human ULBP1-His at 10 μg/ml (100 μl/well) can bind human NKG2D,The EC50 of human NKG2D is 0.39-0.91 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ULBP1 (UL16 Binding Protein 1 (ULBP1))

Alternative Name: ULBP1/N2DL1 (ULBP1 Products)

Synonyms: RAET1I Protein, A430108B07Rik Protein, MULT1 Protein, ULBP1 Protein, UL16 binding protein 1 Protein, UL16 binding protein 21 Protein, NKG2D ligand 1 Protein, ULBP1 Protein, ULBP21 Protein, Ulbp1 Protein, LOC100683099 Protein

Background:

Background: UL16-binding proteins (ULBP) or retinoic acid early transcripts-1 (RAET1) are ligands to the activating receptor, NKG2D. Ten members of the human ULBP/RAET1 gene family have been identified to encode for potentially functional proteins, and have tissue-specific expressions. ULBP1, also known as RAET1I and NKG2DL1, together with at least ULBP 2 and 3, are well-known ligands for NKG2D, and activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. ULBP1 is expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver and bone marrow, as well as some tumor cells. As an unconventional member of the MHC class I family, ULBP1 function in immune responses, especially in cancer and infectious diseases. Unlike other ULBP members, ULBP1 is able to interact with soluble CMV glycoprotein UL16 in CMV infected cells. The interaction with UL16 blocked the interaction with the NKG2D receptor, and thus might escape the immune surveillance. Furthermore, UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. The ULBP1 regulation may have implications for development of new therapeutic strategies against cancer cells.

Synonym: RAET1I

Molecular Weight: 23.8 kDa

NCBI Accession: NP_079494

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process