VEGF 165 (AA 27-191) Protein
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- Target
- VEGF 165
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-191
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human VEGF165 Protein
- Sequence
- Ala27-Arg191
- Characteristics
- Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.It contains Ala27-Arg191.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 0.05 EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 74.2ng/ml determined by ELISA. See testing image for detail.
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VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) protein (His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 207-371) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
VEGF 165 (AA 1-191) protein (His tag)Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
VEGF 165 proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- VEGF 165
- Alternative Name
- VEGF165
- Background
- Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.
- Molecular Weight
- 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.
- UniProt
- P15692-4
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