VEGF 165 (AA 27-191) (Active) protein (His-Avi Tag)

Catalog No. ABIN7275827

Recombinant VEGF 165 protein expressed in HEK-293 Cells.

Quick Overview for VEGF 165 (AA 27-191) (Active) protein (His-Avi Tag) (ABIN7275827)

Target: VEGF 165

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 27-191

Purification tag / Conjugate: His-Avi Tag

Purpose: Non-biotinylated Human VEGF165 Protein

Sequence: Ala27-Arg191

Characteristics: Recombinant Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF165, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA.,Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.,Immobilized Human VEGF165 at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA.,Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody2, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.,Human VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.,Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R1, hFc Tag with an affinity constant of 0.94 nM as determined in BLI assay (Gator® Prime).,Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R2, hFc Tag with an affinity constant of 2.34 nM as determined in BLI assay (Gator® Prime).,Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Anti-VEGF165 Antibody, hFc-Avi Tag with an affinity constant of 31.50 pM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VEGF 165

Alternative Name: VEGF165

Background: Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Molecular Weight: 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

UniProt: P15692