VEGF 165 (AA 27-191) (Active) protein (Biotin,His-Avi Tag)

Catalog No. ABIN7275828

This Recombinant VEGF 165 protein is produced in HEK-293 Cells.

Quick Overview for VEGF 165 (AA 27-191) (Active) protein (Biotin,His-Avi Tag) (ABIN7275828)

Target: VEGF 165

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-191

Purification tag / Conjugate: Biotin,His-Avi Tag

Purpose: Biotinylated Human VEGF165 Protein

Sequence: Ala27-Arg191

Characteristics: Recombinant Biotinylated Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human VEGF165, His Tag captured on CM5 Chip via streptavidin can bind Human VEGF R1, His Tag with an affinity constant of 1.36 pM as determined in SPR assay (Biacore T200).,Immobilized Human VEGF R1, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 10.5ng/ml determined by ELISA.,Immobilized Anti-VEGF165 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 11.6ng/ml determined by ELISA.,Immobilized Human VEGF R2, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 12.6ng/ml determined by ELISA.,Biotinylated VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 90%.,The purity of Biotinylated Human VEGF165 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VEGF 165

Alternative Name: VEGF165

Background: Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Molecular Weight: 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

UniProt: P15692