VEGFC Protein (AA 103-227) (His-Avi Tag)

Catalog No. ABIN7275829

This Recombinant VEGFC protein is expressed in HEK-293 Cells.

Quick Overview for VEGFC Protein (AA 103-227) (His-Avi Tag) (ABIN7275829)

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 103-227

Purification tag / Conjugate: This VEGFC protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human VEGF-C/Flt4-L Protein

Sequence: Thr103-Arg227

Characteristics: Recombinant Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr103-Arg227.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human VEGF R3, His Tag immobilized on CM5 Chip can bind Human VEGF-C, His Tag with an affinity constant of 0.29 nM as determined in SPR assay (Biacore T200).,Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test).,Human VEGF-C on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCI ( pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl ( pH 6.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Alternative Name: VEGF-C

Background: The lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes.

Molecular Weight: 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.

UniProt: Q6FH59

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2