CHI3L1 Protein (AA 30-389) (His tag)

Catalog No. ABIN7275853

This Recombinant CHI3L1 protein is expressed in HEK-293 Cells.

Quick Overview for CHI3L1 Protein (AA 30-389) (His tag) (ABIN7275853)

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 30-389

Purification tag / Conjugate: This CHI3L1 protein is labelled with His tag.

Purpose: Mouse YKL-40/CHI3L1 Protein

Sequence: Tyr30-Ala389

Characteristics: Recombinant Mouse YKL-40/CHI3L1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr30-Ala389.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse YKL-40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse YKL-40 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Alternative Name: YKL-40

Background: YKL-40 (also known as Chitinase 3-like 1) is a glycoprotein produced by inflammatory, cancer and stem cells. Its physiological role is not completely understood but YKL-40 is elevated in the brain and cerebrospinal fluid (CSF) in several neurological and neurodegenerative diseases associated with inflammatory processes. Yet the precise characterization of YKL-40 in dementia cases is missing. YKL-40 is a disease-specific marker of neuroinflammation showing its highest levels in prion diseases. Therefore, YKL-40 quantification might have a potential for application in the evaluation of therapeutic intervention in dementias with a neuroinflammatory component.

Molecular Weight: 41.9 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Bis-Tris PAGE result.

UniProt: Q61362