Cas12a Nuclease (Cpf1) protein (His tag)

Catalog No. ABIN7316792

This Recombinant Cas12a Nuclease protein is expressed in Escherichia coli (E. coli).

Quick Overview for Cas12a Nuclease (Cpf1) protein (His tag) (ABIN7316792)

Target: Cas12a Nuclease (Cpf1)

Protein Type: Recombinant

Origin: Bacteria (Lachnospiraceae)

Source: Escherichia coli (E. coli)

Purity: 95,00 %

Grade: MALS verified

Product Details

Purification tag / Conjugate: His tag

Purpose: GENPower™ NLS-Cas12a Nuclease Protein (MALS verified)

Brand: GENPower™

Sequence: Ser 2 - His 1228

Characteristics: GENPower™ NLS-Cas12a Nuclease is expressed from E.coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).

Endotoxin Level: 0.01 EU per μg

Application Details

Comment:

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 147.6 kDa. The protein migrates as 135-145 kDa under reducing (R) condition (SDS-PAGE). This protein has two NLS, which leads to transport to the nucleus efficiently. It is ideal for in vitro and in vivo reactions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM NaAC,500 mM NaCl,0.1 mM EDTA,0.1 mM TCEP, pH 6.0

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: Cas12a Nuclease (Cpf1)

Alternative Name: Cas12a Nuclease Protein

Background: Synonyms:CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, cas12a, Cpf1,Description:Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Lachnospiraceae bacterium,Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system , an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing. CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector. Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crR, inducing a staggered double-strand break and potential for both R processing and D nuclease activity. In addition, Cas12a is able to process more structured pre-CRISPR/R(crR) molecules into mature crRs which allows the possibility to use both mature or pre-crR for genome editing purposes. All these features make the CRISPR/Cas12a system a valuable genome-engineering tool. CRISPR-Cas12a has been successfully used to edit genomes in many mammalians cells and plants.

Molecular Weight: 147.6 kDa