c-MET Protein (His tag)

Catalog No. ABIN7447886

This Recombinant c-MET protein is produced in HEK-293 Cells.

Quick Overview for c-MET Protein (His tag) (ABIN7447886)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Protein Type: Recombinant

Origin: Rabbit

Source: HEK-293 Cells

Purity: 90,00 %

Grade: MALS verified

Product Details

Purification tag / Conjugate: This c-MET protein is labelled with His tag.

Purpose: Rabbit HGF R / c-MET Protein, His Tag (MALS verified)

Sequence: Glu 25 - Met 933

Characteristics: Rabbit HGF R / c-MET, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Met 933 (Accession # Q09YN5-1).

Endotoxin Level: 1.0 EU per μg

Application Details

Comment:

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 103.3 kDa. The protein migrates as 35-43 kDa and 85-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: HGF R

Background: Synonyms:MET,AUTS9,HGFR,RCCP2,c-Met,Description:Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.

Molecular Weight: 103.3 kDa

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor