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IL-13 Protein (AA 21-132)

This Recombinant IL-13 protein is produced in HEK-293 Cells.
Catalog No. ABIN7454149

Quick Overview for IL-13 Protein (AA 21-132) (ABIN7454149)

Target

See all IL-13 (IL13) Proteins
IL-13 (IL13) (Interleukin 13 (IL13))

Protein Type

Recombinant

Origin

  • 21
  • 15
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Source

  • 29
  • 12
  • 8
  • 7
  • 2
  • 2
  • 1
HEK-293 Cells

Purity

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
  • Protein Characteristics

    AA 21-132

    Purpose

    Human IL-13 Protein

    Sequence

    Gly21-Asn132

    Characteristics

    Recombinant Human IL-13 Protein is expressed from HEK293 without tag.It contains Gly21-Asn132.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized Human IL-13, No Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-13 Ra 2, hFc Tag with the EC50 of 2.84μg/ml determined by ELISA. See testing image for detail.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    Buffer

    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    IL-13 (IL13) (Interleukin 13 (IL13))

    Alternative Name

    IL-13

    Background

    Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

    Molecular Weight

    12.35 kDa. Due to glycosylation, the protein migrates to 20-45 kDa based on Tris-Bis PAGE result.

    Pathways

    JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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