LIF Protein (AA 23-202)

Catalog No. ABIN7454166

Recombinant LIF protein expressed in Escherichia coli (E. coli).

Quick Overview for LIF Protein (AA 23-202) (ABIN7454166)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-202

Purpose: Human LIF Protein

Sequence: Ser23-Phe202

Characteristics: Recombinant Human LIF Protein is expressed from E.coli without tag.It contains Ser23-Phe202.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.15ug/ml determined by ELISA.,Human LIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human LIF is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.

Molecular Weight: 19.86 kDa same as Bis-Tris PAGE result.

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding