LIF Protein (AA 23-202)

Catalog No. ABIN7454166

Recombinant LIF protein expressed in Escherichia coli (E. coli).

Quick Overview for LIF Protein (AA 23-202) (ABIN7454166)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-202

Purpose: Human LIF Protein

Sequence: Ser23-Phe202

Characteristics: Recombinant Human LIF Protein is expressed from E.coli without tag.It contains Ser23-Phe202.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human LIF, His Tag with the EC50 of 81.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.

Molecular Weight: 19.86 kDa same as Tris-Bis PAGE result.

UniProt: P15018-1

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding