SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, B.1.1.7 - alpha, RBD) (Fc Tag)

Catalog No. ABIN7490638

This Recombinant SARS-CoV-2 Spike protein is expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, B.1.1.7 - alpha, RBD) (Fc Tag) (ABIN7490638)

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 319-541, B.1.1.7 - alpha, RBD

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with Fc Tag.

Purpose: Recombinant SARS-CoV-2 S protein RBD(N501Y) protein with C-terminal human Fc tag

Specificity: S protein RBD(N501Y)(Arg319-Phe541) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Alternative Name: SARS-CoV-2 S protein

Target Type: Viral Protein

Background: SARS-CoV-2 B.1.1.7 (Alpha) Spike RBD Protein
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.

Molecular Weight: predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of RBD(N501Y)-hFc is 55-70 kDa due to glycosylation.

UniProt: P0DTC2