RNF43 Protein (AA 43-197) (Fc Tag)

Catalog No. ABIN7490978

Recombinant RNF43 protein expressed in HEK-293 Cells.

Quick Overview for RNF43 Protein (AA 43-197) (Fc Tag) (ABIN7490978)

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 43-197

Purification tag / Conjugate: This RNF43 protein is labelled with Fc Tag.

Purpose: Recombinant human RNF43 protein with C-terminal human Fc tag

Specificity: RNF43(Glu43-Tyr197) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Alternative Name: RNF43

Background: RNF124,SSPCS,URCC
The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Molecular Weight: predicted molecular mass of 39.4 kDa after removal of the signal peptide. The apparent molecular mass of RNF43-hFc is 40-70 kDa due to glycosylation.

UniProt: Q68DV7

Pathways: WNT Signaling