CEACAM8 Protein (AA 35-319) (His tag)

Catalog No. ABIN7491229

This Recombinant CEACAM8 protein is expressed in HEK-293 Cells.

Quick Overview for CEACAM8 Protein (AA 35-319) (His tag) (ABIN7491229)

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 35-319

Purification tag / Conjugate: This CEACAM8 protein is labelled with His tag.

Purpose: Recombinant human CEACAM8 protein with C-terminal 6xHis tag

Specificity: CEACAM8(Gln35-Ser319) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Alternative Name: CEACAM8

Background: CD66b,CD67,CGM6,NCA-95
Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764).[UniProtKB/Swiss-Prot Function]

Molecular Weight: predicted molecular mass of 32.2 kDa after removal of the signal peptide. The apparent molecular mass of CEACAM8-His is 35-70 kDa due to glycosylation.

UniProt: P31997