Angiotensin II Type-1 Receptor Protein (AA 1-27) (Fc Tag)

Catalog No. ABIN7491257

This Recombinant Angiotensin II Type-1 Receptor protein is produced in HEK-293 Cells.

Quick Overview for Angiotensin II Type-1 Receptor Protein (AA 1-27) (Fc Tag) (ABIN7491257)

Target: Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 1-27

Purification tag / Conjugate: This Angiotensin II Type-1 Receptor protein is labelled with Fc Tag.

Purpose: Recombinant Human AGTR1 Protein with C-terminal human Fc tag

Specificity: AGTR1(Met1-Ile27) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))

Alternative Name: AGTR1

Background: AG2S,AGTR1B,AT1,AT1AR,AT1B,AT1BR,AT1R,AT2R1,HAT1R
Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed, however, it is now believed that there is only one type 1 receptor gene in humans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2020]

Molecular Weight: predicted molecular mass of 29.2 kDa after removal of the signal peptide. The apparent molecular mass of AGTR1-hFc is 35-55 kDa due to glycosylation.

UniProt: P30556

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour