OB Cadherin Protein (AA 23-617) (His tag)

Catalog No. ABIN7491301

This Recombinant OB Cadherin protein is expressed in HEK-293 Cells.

Quick Overview for OB Cadherin Protein (AA 23-617) (His tag) (ABIN7491301)

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 23-617

Purification tag / Conjugate: This OB Cadherin protein is labelled with His tag.

Purpose: Recombinant Human CDH11(23-617) Protein with C-terminal 6xHis tag

Specificity: CDH11(Phe23-Thr617) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Alternative Name: CDH11

Background: CAD11,CDHOB,ESWS,OB,OSF-4
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 66.4 kDa after removal of the signal peptide. The apparent molecular mass of CDH11(23-617)-His is 70-100 kDa due to glycosylation.

UniProt: P55287