BTN2A1 Protein (AA 29-248) (His tag)

Catalog No. ABIN7491339

Recombinant BTN2A1 protein expressed in HEK-293 Cells.

Quick Overview for BTN2A1 Protein (AA 29-248) (His tag) (ABIN7491339)

Target: BTN2A1 (butyrophilin, Subfamily 2, Member A1 (BTN2A1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 29-248

Purification tag / Conjugate: This BTN2A1 protein is labelled with His tag.

Purpose: Recombinant Human BTN2A1 Protein with C-terminal 6xHis tag

Specificity: BTN2A1(Gln29-Ala248) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: BTN2A1 (butyrophilin, Subfamily 2, Member A1 (BTN2A1))

Alternative Name: BTN2A1

Background: BK14H9.1,BT2.1,BTF1,BTN2.1,DJ3E1.1
This gene encodes a member of the immunoglobulin superfamily. The gene is located in a cluster of butyrophilin-like genes in the juxta-telomeric region of the major histocompatibility complex on chromosome 6. A pseudogene of this gene has been identified in this cluster. The encoded protein is an integral plasma membrane protein involved in lipid, fatty-acid, and sterol metabolism. Alterations in this gene may be associated with several disease states including metabolic syndrome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Molecular Weight: predicted molecular mass of 25.4 kDa after removal of the signal peptide. The apparent molecular mass of BTN2A1-His is 35-55 kDa due to glycosylation.

UniProt: Q7KYR7

Pathways: Activated T Cell Proliferation