CD47 Protein (CD47) (DYKDDDDK Tag)

Catalog No. ABIN7491584

Synthetic Nanodisc CD47 protein expressed in HEK-293 Cells.

Quick Overview for CD47 Protein (CD47) (DYKDDDDK Tag) (ABIN7491584)

Target: CD47

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Application: ELISA, Immunogen (Imm), Cryogenic electron microscopy (cryo-EM), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Product Details

Purification tag / Conjugate: This CD47 protein is labelled with DYKDDDDK Tag.

Purpose: Human CD47 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD47

Alternative Name: CD47

Background: IAP, MER6, OA3
A membrane protein involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

Molecular Weight: The human full length CD47 protein has a MW of 35.2 kDa

UniProt: Q08722