NGF 7S (Active) Protein

Catalog No. ABIN7887297

This Native NGF 7S protein is produced in Mouse.

Quick Overview for NGF 7S (Active) Protein (ABIN7887297)

Target: NGF 7S

Protein Type: Native

Biological Activity: Active

Origin: Mouse

Source: Mouse

Application: Cell Culture (CC), Cytotoxicity Test (CyTox)

Purity: ≥98 % (HPLC)

Product Details

Purpose: Native mouse NGF 7S protein

Specificity: Toxin target: p75 neurotrophin receptor (p75NTR), TrkA receptors

Biological Activity Comment: NGF is purified in three forms: the 7S, 2.5S and β. The 7S 130 kDa form occurs naturally in mouse submaxillary glands and is a multimeric protein composed of two α, one β and two γ subunits. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical chains held together by hydrophobic interactions. The α chain stabilizes the 7S complex and binds two zinc ions per 7S complex as a cofactor at the β-γ interface. The γ chain is an arginine specific protease, it may also have plasminongen activator activity as well as mitogenic activity for chick embryo fibroblasts1,2.

Application Details

Application Notes: Antigen Preadsorption Control: EC50 = 0.2 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual acetate.

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: NGF 7S

Background: A native mouse nerve growth factor (NGF) 7S protein complex that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors

Molecular Weight: 130 kDa

CAS-No: 93928-24-6