Nerve Growth Factor Protein (NGF)

Catalog No. ABIN7491868

This Recombinant Nerve Growth Factor protein is produced in Escherichia coli (E. coli).

Quick Overview for Nerve Growth Factor Protein (NGF) (ABIN7491868)

Target: Nerve Growth Factor (NGF)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC), Cytotoxicity Test (CyTox)

Purity: ≥98 % (HPLC)

Product Details

Purpose: Recombinant mouse proNGF protein

Sequence: MEPYTDSNVP EGDSVPEAHW TKLQHSLDTA LRRARSAPTA PIAARVTGQT RNITVDPRLF KKRRLHSPRV LFSTQPPPTS SDTLDLDFQA HGTIPFNRTH RSKRSSTHPV FHMGEFSVCD SVSVWVGDKT TATDIKGKEV TVLAEVNINN SVFRQYFFET KCRASNPVES GCRGIDSKHW NSYCTTTHTF VKALTTDEKQ AAWRFIRIDT ACVCVLSRKA TRRG (the prodomain is shown in bold)

Specificity: Toxin target: p75 neurotrophin receptor (p75NTR) and VPS10 domain-containing receptors (Sortilin and SorCS2)

Endotoxin Level: <0.1 EU per 1 μg of the protein by the LAL method.

Biological Activity Comment: proNGF is the preferred ligand of p75NTR 1. In contrast to its mature form proNGF induces apoptosis via p75NTR 1, but can also mediate cell survival in DRGs2.

Application Details

Application Notes: Antigen Preadsorption Control: 0.4 - 4 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: Nerve Growth Factor (NGF)

Alternative Name: proNGF

Background: A recombinant mouse proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis

Molecular Weight: 50.4 kDa (dimer)

Pathways: Regulation of Cell Size