CD34 Protein (CD34) (Biotin,His-Avi Tag)
Quick Overview for CD34 Protein (CD34) (Biotin,His-Avi Tag) (ABIN7489026)
Target
See all CD34 ProteinsProtein Type
Origin
Source
Purity
Grade
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Purification tag / Conjugate
- This CD34 protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Human CD34 Protein, His,Avitag™ (MALS verified)
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Sequence
- Ser 32 - Thr 290
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Characteristics
- Biotinylated Human CD34 Protein, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Thr 290 (Accession # P28906-1).
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Endotoxin Level
- 1.0 EU per μg
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CD34 (CD34) (AA 35-287) protein (His tag)
CD34 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD34 (CD34) (AA 32-290) protein (His tag)CD34 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD34 (CD34) (AA 124-225) protein (His tag)CD34 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CD34 (CD34) (AA 32-290) protein (His-IF2DI Tag)CD34 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CD34 (CD34) (AA 32-141) protein (GST tag)CD34 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
CD34 (CD34) (AA 1-328) protein (GST tag)CD34 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
CD34 (CD34) (AA 35-287) protein (Fc Tag)CD34 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD34 (CD34) protein (Fc Tag)CD34 Origin: Rat Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
CD34 (CD34) (AA 32-290) protein (His tag)CD34 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
CD34 (CD34) (Active) protein (His tag)CD34 Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
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Comment
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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). (Biotin,10xHis , Avi) The protein has a calculated MW of 31.1 kDa. The protein migrates as 50-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- PBS, pH 7.4
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Storage
- -20 °C
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Storage Comment
- -20°C
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- CD34
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Alternative Name
- CD34
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Background
- CD34 Molecule is a cluster of differentiation molecule present on certain cells within the human body. It is a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. As a ~ 110 kDa monomeric cell surface antigen, CD34 is highly glycosylated with nine potential N-linked and numerous potential O-linked glycosylation sites in its extracellular domain. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia whereas the L-selectin to which it binds is on the T cell. It was indicated that CD34 is a phosphorylation target for activated PKC, and couples to the hematopoietic adapter protein CrkL, which were involved in CD34 signaling pathways. CD34 is abberantly expressed in many kinds of tumors and is implicated in leukemogenesis.
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Molecular Weight
- 31.1 kDa
Target
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