Alkaline Phosphatase Protein (ALP) (AA 22-510) (His tag)
Quick Overview for Alkaline Phosphatase Protein (ALP) (AA 22-510) (His tag) (ABIN7504230)
Target
Protein Type
Origin
Source
Purity
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Protein Characteristics
- AA 22-510
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Purification tag / Conjugate
- This Alkaline Phosphatase protein is labelled with His tag.
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Purpose
- Rat Alkaline Phosphatase (Germ type) /ALPG Protein
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Sequence
- Val22-Pro510
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Characteristics
- Recombinant Rat Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val22-Pro510.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- Alkaline Phosphatase (ALP)
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Alternative Name
- Alkaline Phosphatase
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Background
- Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
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Molecular Weight
- 54.10 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
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UniProt
- F1M8U7
Target
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