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Alkaline Phosphatase Protein (ALP) (AA 22-510) (His tag)

This Recombinant Alkaline Phosphatase protein is produced in HEK-293 Cells.
Catalog No. ABIN7504230

Quick Overview for Alkaline Phosphatase Protein (ALP) (AA 22-510) (His tag) (ABIN7504230)

Target

Alkaline Phosphatase (ALP)

Protein Type

Recombinant

Origin

  • 7
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  • 1
Rat

Source

  • 7
  • 2
  • 1
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HEK-293 Cells

Purity

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
  • Protein Characteristics

    AA 22-510

    Purification tag / Conjugate

    This Alkaline Phosphatase protein is labelled with His tag.

    Purpose

    Rat Alkaline Phosphatase (Germ type) /ALPG Protein

    Sequence

    Val22-Pro510

    Characteristics

    Recombinant Rat Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val22-Pro510.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1EU per μg by the LAL method.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    Buffer

    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Alkaline Phosphatase (ALP)

    Alternative Name

    Alkaline Phosphatase

    Background

    Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

    Molecular Weight

    54.10 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

    UniProt

    F1M8U7
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