CD177 Protein (AA 26-520) (Biotin,His-Avi Tag)

Catalog No. ABIN7504256

Recombinant CD177 protein expressed in HEK-293 Cells.

Quick Overview for CD177 Protein (AA 26-520) (Biotin,His-Avi Tag) (ABIN7504256)

Target: CD177 (CD177 Molecule (CD177))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-520

Purification tag / Conjugate: This CD177 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human CD117 Protein

Sequence: Gln26-Pro520

Characteristics: Recombinant Biotinylated Human CD117 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Pro520.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD117, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).,Immobilized Human SCF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8ug/ml determined by ELISA.,Biotinylated Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CD117 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177

Background: The stem cell factor receptor (SCFR, CD117) is encoded by the c-kit proto-oncogene, which is the cellular counterpart of the v-kit oncogene of a transforming feline retrovirus (HZ4-feline sarcoma virus). It is a 145kD transmembrane glycoprotein which is homologous to several other growth factor receptors such as the receptors for macrophage colony stimulating factor (M-CSF, CSF-1, c-fms), platelet-derived growth factor a and b (PDGF-Ra , PDGF-Rb ), and the newly described growth factor receptor STK-1, which is the human homologue of the murine FLK2/FLT3 receptor.

Molecular Weight: 58.60 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.

UniProt: P10721