FGF8 Protein (AA 23-204)

Catalog No. ABIN7504328

Recombinant FGF8 protein expressed in Escherichia coli (E. coli).

Quick Overview for FGF8 Protein (AA 23-204) (ABIN7504328)

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 23-204

Purpose: Human FGF8a Protein

Sequence: Gln23-Arg204

Characteristics: Recombinant Human FGF8a Protein is expressed from E.coli without tag.It contains Gln23-Arg204.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.01 EU per μg by the LAL method.

Biological Activity Comment: Human FGF8a-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50-for this effect is 0.5 - 2 ug/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 20 mM Tris-HCl, 300 mM NaCl ( pH 8.5). Normally 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Alternative Name: FGF8a

Background: Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.

Molecular Weight: 21.20 kDa same as Bis-Tris PAGE result.

UniProt: P55075

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis