Sca-1/Ly-6A/E Protein (AA 27-119) (His tag)

Catalog No. ABIN7504459

This Recombinant Sca-1/Ly-6A/E protein is expressed in Escherichia coli (E. coli).

Quick Overview for Sca-1/Ly-6A/E Protein (AA 27-119) (His tag) (ABIN7504459)

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-119

Purification tag / Conjugate: This Sca-1/Ly-6A/E protein is labelled with His tag.

Purpose: Mouse LY6A Protein

Sequence: Leu27-Gly119

Characteristics: Recombinant Mouse LY6A Protein is expressed from E.coli with His tag at the N-terminus.It contains Leu27-Gly119.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse LY6A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse LY6A is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: LY6A

Background: Stem Cell Antigen-1 (Sca-1/Ly6A) was the first identified member of the Lymphocyte antigen-6 (Ly6) gene family. Sca-1 is a cell surface glycoprotein that is often used as a biomarker for stem cells and cell stemness.

Molecular Weight: 10.80 kDa. The protein migrates to 15-20 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_001258345

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation