CSF1R Protein (AA 20-512)

Catalog No. ABIN7504465

This Recombinant CSF1R protein is expressed in HEK-293 Cells.

Quick Overview for CSF1R Protein (AA 20-512) (ABIN7504465)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-512

Purpose: Human M-CSF R/CSF1R/CD115 Protein

Sequence: Ile20-Glu512

Characteristics: Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 without tag.It contains Ile20-Glu512.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human M-CSF R, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).,Human M-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human M-CSF, His Tag on Anti-His-Biosensor can bind Human M-CSF R, No Tag with an affinity constant of 0.46 μM as determined in BLI assay (Gator® Prime).,The purity of Human M-CSF R is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: M-CSF R

Background: CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Molecular Weight: 54.49 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.

UniProt: P07333

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization