SKP1 Protein (AA 2-163) (AVI tag,Biotin)

Catalog No. ABIN7504529

Recombinant SKP1 protein expressed in HEK-293 Cells.

Quick Overview for SKP1 Protein (AA 2-163) (AVI tag,Biotin) (ABIN7504529)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 2-163

Purification tag / Conjugate: This SKP1 protein is labelled with AVI tag,Biotin.

Purpose: Biotinylated Human SKP1 Protein

Sequence: Pro2-Lys163

Characteristics: Recombinant Biotinylated Human SKP1 Protein is expressed from HEK293 His tag at the N-terminus and Avi tag at the C-terminusIt contains Pro2-Lys163.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1

Background: The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes.

Molecular Weight: 21.43 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.

UniProt: P63208-1

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases