PLAT Protein (AA 33-559) (hFc Tag)

Catalog No. ABIN7504553

Recombinant PLAT protein expressed in HEK-293 Cells.

Quick Overview for PLAT Protein (AA 33-559) (hFc Tag) (ABIN7504553)

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 33-559

Purification tag / Conjugate: This PLAT protein is labelled with hFc Tag.

Purpose: Mouse tPA Protein

Sequence: Ser33-Gln559

Characteristics: Recombinant Mouse tPA Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Ser33-Gln559, which consists of two chains: chain A (Ser36-Arg308) and chain B (Ile309-Gln559).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse tPA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is > 200 pmol/min/ug.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Alternative Name: tPA

Background: Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.

Molecular Weight: 85.00 kDa. It migrates to 35-40 kDa and 30-35 kDa based on Bis-Tris PAGE result.

UniProt: P11214

Pathways: Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome