Caspase 1 Protein (CASP1) (AA 120-296) (His tag)

Catalog No. ABIN7505395

This Recombinant Caspase 1 protein is produced in Escherichia coli (E. coli).

Quick Overview for Caspase 1 Protein (CASP1) (AA 120-296) (His tag) (ABIN7505395)

Target: Caspase 1 (CASP1)

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 120-296

Purification tag / Conjugate: This Caspase 1 protein is labelled with His tag.

Sequence: Asn 120-Asp 296

Characteristics: A DNA sequence encoding the Mouse CASP1 protein (P29452) (Asn 120-Asp 296) was expressed with a N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Caspase 1 (CASP1)

Alternative Name: CASP1

Background:

Abbreviation: CASP1

Target Synonym: CASP-1,CASP1,CASP1,Caspase 1,Caspase-1 subunit p10,ICE,IL-1 beta-converting enzyme,IL-1BC

Background: Caspase-1, also known as IL-1 beta -converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50 kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits.

Molecular Weight:

Calculated MW: 19.36 kDa

Observed MW: 21 kDa

UniProt: P29452

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome