GFP Protein (AA 1-238)

Catalog No. ABIN7505456

This Recombinant GFP protein is expressed in Escherichia coli (E. coli).

Quick Overview for GFP Protein (AA 1-238) (ABIN7505456)

Target: GFP (Green Fluorescent Protein (GFP))

Protein Type: Recombinant

Origin: Aequorea victoria

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-238

Sequence: Met1-Lys238

Characteristics: A DNA sequence encoding the Aequorea victoria GFP Protein(P42212)(1-238)was expressed with a polyhistidine tag at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % tween-80.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background:

Abbreviation: GFP

Target Synonym: Green fluorescent protein

Background: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Molecular Weight:

Calculated MW: 26.9 kDa

Observed MW: 30 kDa

UniProt: P42212