GCK Protein (AA 1-147) (His tag)

Catalog No. ABIN7505499

Recombinant GCK protein expressed in Escherichia coli (E. coli).

Quick Overview for GCK Protein (AA 1-147) (His tag) (ABIN7505499)

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-147

Purification tag / Conjugate: This GCK protein is labelled with His tag.

Sequence: Met 1-Gly 147

Characteristics: A DNA sequence encoding the Rat GCK protein (P17712) (Met 1-Gly 147) was expressed with a N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: GCK

Background:

Abbreviation: GCK

Target Synonym: GCK,Glucokinase,Hexokinase-4,Hexokinase 4

Background: Hexokinases phosphorylate hexose to form hexose 6?phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucose kinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose?6?phosphate, GCK has much lower affinity for glucose and is not inhibited by the product. Consequently, GCK has a Km for glucose of approximately 7 mM, which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells. In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non?insulin?dependent diabetes mellitus, maturity?onset diabetes of the young type 2, and hyperinsulinemia of infancy. The enzyme activity was measured using a phosphatase coupled kinase assay.

Molecular Weight:

Calculated MW: 16.06 kDa

Observed MW: 16 kDa

UniProt: P17712

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process