AIF Protein (AA 150-299) (His tag)

Catalog No. ABIN7505526

Recombinant AIF protein expressed in Escherichia coli (E. coli).

Quick Overview for AIF Protein (AA 150-299) (His tag) (ABIN7505526)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 150-299

Purification tag / Conjugate: This AIF protein is labelled with His tag.

Sequence: Arg 150-Val 299

Characteristics: A DNA sequence encoding the Rat AIF-M1 protein (Q9JM53) (Arg 150-Val 299) was expressed with a N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIF-M1

Background:

Abbreviation: AIF-M1

Target Synonym: AIFM1,Apoptosis inducing factor 1,mitochondrial,Apoptosis inducing factor,CMTX4,COWCK,COXPD6,Harlequin,mitochondrial,PDCD 8,Programmed cell death 8,Striatal apoptosis inducing factor

Background: Probable oxidoreductase that has a dual role in controlling cellular life and death, during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Molecular Weight:

Calculated MW: 16.39 kDa

Observed MW: 18 kDa

UniProt: Q9JM53

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect