MICA Protein (AA 1-308) (His tag)

Catalog No. ABIN7505607

Recombinant MICA protein expressed in HEK-293 Cells.

Quick Overview for MICA Protein (AA 1-308) (His tag) (ABIN7505607)

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-308

Purification tag / Conjugate: This MICA protein is labelled with His tag.

Sequence: Met1-Gln308

Characteristics: A DNA sequence encoding theHuman MICA protein (Q96QC4) (Met1-Gln308) was expressed with a C-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA

Background:

Abbreviation: MICA

Target Synonym: MHC class I chain-related protein A,Stress inducible class I homolog,MHC class I polypeptide-related sequence A

Background: MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to the MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules do not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gamma-delta T cells, CD8+ alpha-beta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by the ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gamma-delta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.

Molecular Weight:

Calculated MW: 33 kDa

Observed MW: 50-60 kDa

UniProt: Q96QC4

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response