CD14 Protein (CD14) (His tag)
Quick Overview for CD14 Protein (CD14) (His tag) (ABIN7534605)
Target
See all CD14 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This CD14 protein is labelled with His tag.
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Purpose
- Active Recombinant Human CD14 Protein
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Sequence
- TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV PAC
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Specificity
- Thr20-Cys352
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 5.7-23 ng/mL.
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CD14 (CD14) (AA 16-345) protein (His tag)
CD14 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 20-344) protein (His tag)CD14 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 1-375) protein (GST tag)CD14 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD14 (CD14) (AA 20-352) protein (His tag)CD14 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD14 (CD14) (AA 20-349) protein (His tag)CD14 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
CD14 (CD14) protein (His tag,Fc Tag)CD14 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD14 (CD14) protein (Fc Tag)CD14 Origin: Rat Host: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- CD14
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Alternative Name
- CD14
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Background
- CD14
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Gene ID
- 929
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UniProt
- P08571
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Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Target
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