PD-L1 Protein (His tag)

Catalog No. ABIN7535911

Recombinant PD-L1 protein expressed in HEK-293 Cells.

Quick Overview for PD-L1 Protein (His tag) (ABIN7535911)

Target: PD-L1 (CD274 (PD-L1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This PD-L1 protein is labelled with His tag.

Purpose: Active Recombinant Rat B7-H1/PD-L1/CD274 Protein

Sequence: AFTITAPKDL YVVEYGSNVT MECRFPVEQK LDLLALVVYW EKEDKEVIQF VEGEEDLKPQ HSSFRGRAFL PKDQLLKGNA VLQITDVKLQ DAGVYCCMIS YGGADYKRIT LKVNAPYRKI NQRISMDPAT SEHELMCQAE GYPEAEVIWT NSDHQSLSGE TTVTTSQTEE KLLNVTSVLR VNATANDVFH CTFWRVHSGE NHTAELIIPE LPVPRLPHNR T

Specificity: Ala18-Thr238

Sterility: 0.22 μm filtered

Endotoxin Level: <0.1EU/μg

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Rat PD-L1 at 10 μg/mL (100 μL/well) can bind Biotinylated Rat PD-1 with a linear range of 0.0195-2.16 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: B7-H1/PD-L1/CD274

Background:

Description: This protein is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation.

Name: Pdcd1lg1, RGD1566211,CD274

Gene ID: 499342

UniProt: D4AE25

Pathways: Cancer Immune Checkpoints