CD276 Protein (CD276) (His tag)
Quick Overview for CD276 Protein (CD276) (His tag) (ABIN7534652)
Target
See all CD276 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This CD276 protein is labelled with His tag.
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Purpose
- Active Recombinant Human B7-H3/CD276 Protein
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Sequence
- LEVQVPEDPV VALVGTDATL CCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FAEGQDQGSA YANRTALFPD LLAQGNASLR LQRVRVADEG SFTCFVSIRD FGSAAVSLQV AAPYSKPSMT LEPNKDLRPG DTVTITCSSY RGYPEAEVFW QDGQGVPLTG NVTTSQMANE QGLFDVHSVL RVVLGANGTY SCLVRNPVLQ QDAHGSVTIT GQPMTFP
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Specificity
- Leu29-Pro245
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human B7-H3 at 2 μg/mL (100 μL/well) can bind Anti-Human B7-H3 Antibody with a linear range of 1-4 ng/mL.
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CD276 (CD276) (AA 29-245) protein (His tag)
CD276 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD276 (CD276) (AA 27-461) protein (Fc Tag)CD276 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-245) protein (mFc-His Tag)CD276 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD276 (CD276) (AA 29-245) protein (His-Avi Tag,Biotin)CD276 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-248) protein (His tag)CD276 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-244) protein (Fc Tag)CD276 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD276 (CD276) (AA 28-140) protein (His-DYKDDDDK-Strep II Tag)CD276 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD276 (CD276) (AA 237-461) protein (His-DYKDDDDK-Strep II Tag)CD276 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD276 (CD276) (AA 28-238) protein (His-DYKDDDDK-Strep II Tag)CD276 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD276 (CD276) (AA 29-245) protein (Fc Tag,AVI tag,Biotin)CD276 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- CD276
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Alternative Name
- B7-H3/CD276
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Background
- CD276, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, CD276 antigen,4Ig-B7-H3,B7-H3,B7H3,B7RP-2
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Gene ID
- 80381
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UniProt
- Q5ZPR3-2
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Pathways
- Cancer Immune Checkpoints
Target
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