Chitotriosidase 1 Protein (His tag)
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- Target See all Chitotriosidase 1 (CHIT1) Proteins
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Chitotriosidase 1 protein is labelled with His tag.
- Purpose
- Active Recombinant Human Chitinase-1/CHIT1 Protein
- Sequence
- AKLVCYFTNW AQYRQGEARF LPKDLDPSLC THLIYAFAGM TNHQLSTTEW NDETLYQEFN GLKKMNPKLK TLLAIGGWNF STQKFTDMVA TANNRQTFVN SAIRFLRKYS FDGLDLDWEY PGSQGSPAVD KERFTTLVQD LANAFQQEAQ TSGKERLLLS AAVPAGQTYV DAGYEVDKIA QNLDFVNLMA YDFHGSWEKV TGHNSPLYKR QEESGAAASL NVDAAVQQWL QKGTPASKLI LGMPTYGRSF TLASSSDTRV GAPATGSGTP GPFTKEGGML AYYEVCSWKG ATKQRIQDQK VPYIFRDNQW VGFDDVESFK TKVSYLKQKG LGGAMVWALD LDDFAGFSCN QGRYPLIQTL RQELSLPYLP SGTPELEVPK PGQPSEPEHG PSPGQDTFCQ GKADGLYPNP RERSSFYSCA GGRLFQQSCP TGLVFSNSCK CCTWN
- Specificity
- Ala22-Asn466
- Purity
- > 95 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.01 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its ability to cleave 4-Methylumbelliferyl-beta -D-N,N',N""-triacetylchitotriose (4-MU-TACT). The specific activity is >43571 pmol/min/μg.
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Chitotriosidase (CHIT1) (AA 1-466) protein (GST tag)
CHIT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chitotriosidase (CHIT1) protein (Myc-DYKDDDDK Tag)CHIT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- Alternative Name
- Chitinase-1/CHIT1 (CHIT1 Products)
- Background
- CHIT1,CHI3,CHIT,CHITD
- Gene ID
- 1118
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